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(A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and <t>DAPI</t> (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.
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Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
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Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
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Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
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Image Search Results


(A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and DAPI (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.

Journal: PLOS One

Article Title: Transcranial photobiomodulation therapy with 808 nm light changes expression of genes and proteins associated with neuroprotection, neuroinflammation, oxidative stress, and Alzheimer’s disease: Whole RNA sequencing of mouse cortex and hippocampus

doi: 10.1371/journal.pone.0326881

Figure Lengend Snippet: (A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and DAPI (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.

Article Snippet: The tissue slices were stained with Arginase 1 antibody, inducible nitric oxide synthase (iNOS) antibody [ ], and DAPI fluorescent probe (Thermo Fisher Scientific, USA).

Techniques: Fluorescence, Microscopy, Staining

Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Orally Administered Functional Polyphenol-Nanozyme-Armored Probiotics for Enhanced Amelioration of Intestinal Inflammation and Microbiota Dysbiosis.

doi: 10.1002/advs.202411939

Figure Lengend Snippet: Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.

Article Snippet: 3,3′,5,5′-Tetramethylbenzidine (TMB: ST746), DCFH-DA fluorescent probe, Total SOD Assay Kit with WST-8 (Cat# S0101S), Catalase Assay Kit (Cat# S0051), Hoechst 33342 (Cat# C1028), and DAPI fluorescent probe were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Activity Assay, Staining, Enzyme-linked Immunosorbent Assay